Membrane filter contact technique for bacteriological sampling of moist surfaces

A technique for bacteriological sampling of hair.

Bacteriological sampling of hair has been previously carried out by applying culture plates directly to the hair but the resulting heavy growth of mixed organisms could make rapid identification of individual colonies difficult and the use of selective media with this technique prevents a comparison of relative numbers of different organisms. The method described here, based onatechnique used b...

Bacteriological Technique

Fine-needle aspiration, an efficient sampling technique for bacteriological diagnosis of nonulcerative Buruli ulcer.

Invasive punch or incisional skin biopsy specimens are currently employed for the bacteriological confirmation of the clinical diagnosis of Buruli ulcer (BU), a cutaneous infectious disease caused by Mycobacterium ulcerans. The efficacy of fine-needle aspirates (FNA) using fine-gauge needles (23G by 25 mm) for the laboratory confirmation of BU was compared with that of skin tissue fragments obt...

Membrane filter technique for the isolation of Yersinia enterocolitica.

A membrane filter procedure was developed for the isolation of Yersinia enterocolitica from aquatic environments. Primary differentiation was based on the fermentation of sorbitol, the absence of lysine decarboxylase and arginine decarboxylase-dihydrolase activities, and the production of urease. Sodium deoxycholate was incorporated as an inhibitor of background organisms. The presumptive ident...

A membrane-filter technique for the detection of complementary DNA.